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blunt ta ligase master mix  (New England Biolabs)


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    Structured Review

    New England Biolabs blunt ta ligase master mix
    Blunt Ta Ligase Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blunt+ta+ligase+master+mix/Blunt%2FTA+Ligase+Master+Mix/10__1038_slash_s41598___026___74202___2-97-28-27
    Average 99 stars, based on 1158 article reviews
    blunt ta ligase master mix - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
    Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

    Ligation:

    Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
    Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

    Multiplexing:

    Article Title: NanoTS: a deep learning tool for accurate SNP calling in nanopore long-read transcriptome data.
    Article Snippet: Briefly, 120 ng of cDNA was repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-tailing Module (NEB, cat. no. E7546L) followed by purification with DNA Clean Beads. .. For multiplexing, the repaired DNA was barcoded using the Native Barcode kit with NEB Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) and purified again with DNA Clean Beads. ..

    Purification:

    Article Title: NanoTS: a deep learning tool for accurate SNP calling in nanopore long-read transcriptome data.
    Article Snippet: Briefly, 120 ng of cDNA was repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-tailing Module (NEB, cat. no. E7546L) followed by purification with DNA Clean Beads. .. For multiplexing, the repaired DNA was barcoded using the Native Barcode kit with NEB Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) and purified again with DNA Clean Beads. ..

    Article Title: Host exonuclease SbcB and a phage-encoded SSB-like protein control activation of the DRT10 reverse transcriptase defense system
    Article Snippet: An anchored primer (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTTTTTTTTTV-3’) was annealed to the poly-A tails and extended with exo- Klenow polymerase (New England Biolabs). .. Reaction products were purified using QIAquick PCR cleanup columns (Qiagen) and ligated to pre-annealed adapters (top strand: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGT-3’; bottom strand: 5’- /5Phos/CTGTCTCTTATACACATCTGACGCTGCCGACGA/3AmMO/-3’) using Blunt/TA Ligase Master Mix (New England Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: Host exonuclease SbcB and a phage-encoded SSB-like protein control activation of the DRT10 reverse transcriptase defense system
    Article Snippet: An anchored primer (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTTTTTTTTTV-3’) was annealed to the poly-A tails and extended with exo- Klenow polymerase (New England Biolabs). .. Reaction products were purified using QIAquick PCR cleanup columns (Qiagen) and ligated to pre-annealed adapters (top strand: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGT-3’; bottom strand: 5’- /5Phos/CTGTCTCTTATACACATCTGACGCTGCCGACGA/3AmMO/-3’) using Blunt/TA Ligase Master Mix (New England Biolabs). ..



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